Postdoc in the Charbonnier Lab - Division of Immunology, Boston Children's

A postdoctoral position is available for an enthusiastic scientist to work in the area of regulatory T cell immunology. The position is located at the division of Immunology, Boston Children’s Hospital and Harvard Medical School under the supervision of Dr. Louis-Marie Charbonnier. This lab focuses on elucidating the mechanisms involved in the breakdown of immune tolerance in monogenic disorders of immunity, autoimmune diseases and allergic inflammatory disorders, with a particular focus on the role of regulatory T cells in disease pathogenesis. More specifically, the projects involve the regulatory circuitries underlying Treg cell fate decisions, Treg cell heterogeneity and reprogramming as well as metabolic and molecular therapeutics to promote immune tolerance, in the context of autoimmunity, Hyper IgE syndrome and Primary Immunodeficiencies.

Relevant articles :

1- Charbonnier LM, Wang S, Georgiev P, Sefik E and Chatila TA. Control of Peripheral Tolerance by Regulatory T Cell- Intrinsic Notch signaling. Nat. Immunol. 2015 Nov;16(11):1162-73

2- Charbonnier LM, Cui Y, Stephen-Victor E, Harb H, Lopez D, Bleesing JJ, Garcia-Lloret MI, Chen K, Ozen A, Carmeliet P, Li MO, Pellegrini M, Chatila TA. Functional reprogramming of regulatory T cells in the absence of Foxp3. Nat Immunol. 2019 Sep;20(9):1208-1219

3- Zemmour D, Charbonnier LM, Leon J, Six E, Keles S, Delville M, Benamar M, Baris S, Zuber J, Chen K, Neven B, Garcia- Lloret MI, Ruemmele F, Brugnara C, Cerf-Bensussan N, Rieux-Laucat F, Cavazzana M, André I, Chatila TA, Mathis D, Benoist C. Single cell analysis of FOXP3 deficiencies in humans and mice unmasks intrinsic and extrinsic CD4+ T cell perturbations. Nat Immunol. 2021 May;22(5):607-619.

4- Abdel-Gadir A, Stephen-Victor E, Gerber GK, Noval Rivas M, Wang S, Harb H, Wang L, Li N, Crestani E, Spielman S, Secor W, Biehl H, DiBenedetto N, Dong X, Umetsu DT, Bry L, Rachid R, Chatila TA. Microbiota therapy acts via a regulatory T cell MyD88/RORγt pathway to suppress food allergy. Nat Med 2019 Jul;25(7):1164-1174.

This position is ideal for an individual with high interest in high throughput methodologies (scRNA-seq, ChIP-seq, ATAC-seq) to study basic and translational Treg cell immunoregulatory functions and development. Candidates are expected to be independent and self-motivated with experience and/or knowledge of contemporary high-throughput sequencing techniques as well as immunological mouse models, human blood sample processing as well as cell culture, flow cytometry and cell sorting.Candidates must hold a doctoral degree (PhD, MD, or the equivalent) in molecular, cell biology, immunology or related fields. Those with experience in Primary Immunodeficiencies, mouse models of immunology, mammalian cell isolation, cell culture, flow cytometry, in vitro gene manipulation, RNA isolation and handling, hands-on expertise in molecular biology and transcription regulation are highly encouraged to apply. Performing high-throughput sequencing library preparation is highly desirable. In addition, strong oral and written communication skills, excellent organizational and recordkeeping skills with high attention to detail, the ability to work independently as well as collaboratively with others, personal and professional integrity and flexibility in work scheduling are highly valued.

To apply, interested individuals should submit their CV, a cover letter outlining interests and accomplishments to Dr. Louis- Marie Charbonnier : Louis-Marie.Charbonnier (at) childrens.harvard.edu

Salary will follow NIH guidelines commensurate with training and experience. Boston Children’s Hospital, Harvard Medical School is an Affirmative Action/Equal Opportunity Employer.